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验证数据展示

  • WB analysis using 22393-1-AP (same clone as 22393-1-PBS)

    WB analysis using 22393-1-AP (same clone as 22393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22393-1-AP (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 22393-1-AP (same clone as 22393-1-PBS)

    WB analysis of HEK-293 using 22393-1-AP (same clone as 22393-1-PBS)

    WB result of MSH3 antibody (22393-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 22393-1-AP (same clone as 22393-1-PBS)

    IP experiment of HeLa using 22393-1-AP (same clone as 22393-1-PBS)

    IP result of anti-MSH3 (IP:22393-1-AP, 4ug; Detection:22393-1-AP 1:1000) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 22393-1-AP (same clone as 22393-1-PBS)

    IF Staining of HT-29 using 22393-1-AP (same clone as 22393-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MSH3 antibody (22393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MSH3 Polyclonal antibody, PBS Only

MSH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22393-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

mutS homolog 3, Mismatch repair protein 1, hMSH3, DUG, DUC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

22393-1-PBS targets MSH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 MSH3 fusion protein Ag18060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mutS homolog 3 (E. coli)
别名 mutS homolog 3, Mismatch repair protein 1, hMSH3, DUG, DUC1
计算分子量 1137 aa, 127 kDa
观测分子量 127 kDa
GenBank蛋白编号BC130434
基因名称 MSH3
Gene ID (NCBI) 4437
RRIDAB_11232405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20585
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).