验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, mouse liver tissue |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27339-1-AP targets MRPL35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | MRPL35 fusion protein Ag26300 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | mitochondrial ribosomal protein L35 |
别名 | MRPL 35, MRP L35, L35mt |
计算分子量 | 170 aa, 19 kDa |
观测分子量 | 24 kDa |
GenBank蛋白编号 | BC020651 |
基因名称 | MRPL35 |
Gene ID (NCBI) | 51318 |
RRID | AB_3669593 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NZE8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.
实验方案
Product Specific Protocols | |
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WB protocol for MRPL35 antibody 27339-1-AP | Download protocol |
IF protocol for MRPL35 antibody 27339-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |