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验证数据展示

  • WB analysis using 27339-1-AP

    WB analysis using 27339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27339-1-AP (MRPL35 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27339-1-AP

    IHC staining of mouse liver using 27339-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27339-1-AP (MRPL35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 27339-1-AP

    IF Staining of HepG2 using 27339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL35 antibody (27339-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MRPL35 Polyclonal antibody

MRPL35 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27339-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

MRPL 35, MRP L35, L35mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27339-1-AP targets MRPL35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MRPL35 fusion protein Ag26300 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L35
别名 MRPL 35, MRP L35, L35mt
计算分子量 170 aa, 19 kDa
观测分子量24 kDa
GenBank蛋白编号BC020651
基因名称 MRPL35
Gene ID (NCBI) 51318
RRIDAB_3669593
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZE8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.

实验方案

Product Specific Protocols
WB protocol for MRPL35 antibody 27339-1-APDownload protocol
IF protocol for MRPL35 antibody 27339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols