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验证数据展示

  • WB analysis using 26454-1-AP

    WB analysis using 26454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26454-1-AP (MOS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26454-1-AP

    IF Staining of HeLa using 26454-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MOS antibody (26454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 26454-1-AP

    IHC staining of human liver using 26454-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26454-1-AP

    IHC staining of human liver using 26454-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 40x lens).

MOS Polyclonal antibody

MOS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26454-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

c-mos, MOS, MSV, oncogene MOS, Oocyte maturation factor mos, Proto oncogene c Mos

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26454-1-AP targets MOS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MOS fusion protein Ag24585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-mos Moloney murine sarcoma viral oncogene homolog
别名 c-mos, MOS, MSV, oncogene MOS, Oocyte maturation factor mos, Proto oncogene c Mos
计算分子量 346 aa, 38 kDa
观测分子量 34-38 kDa
GenBank蛋白编号BC069569
基因名称 MOS
Gene ID (NCBI) 4342
RRIDAB_3085874
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mos is a germ cell-specific serine/threonine protein kinase, can induce oncogenic transformation of somatic cells by direct phosphorylation of MAP kinase/ERK kinase (MEK1), activating the mitogen-activated protein kinases ERK1 and ERK2.

实验方案

Product Specific Protocols
WB protocol for MOS antibody 26454-1-APDownload protocol
IHC protocol for MOS antibody 26454-1-APDownload protocol
IF protocol for MOS antibody 26454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols