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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14552-1-AP (same clone as 14552-1-PBS)

    WB analysis using 14552-1-AP (same clone as 14552-1-PBS)

    A 549 cell lysates were subjected to SDS PAGE followed by western blot with 14552-1-AP (MMP14 / MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14552-1-AP (same clone as 14552-1-PBS)

    IF Staining of HepG2 using 14552-1-AP (same clone as 14552-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP14 / MT1-MMP antibody (14552-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14552-1-PBS in a different storage buffer formulation.

MMP14 / MT1-MMP Polyclonal antibody, PBS Only

MMP14 / MT1-MMP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 14552-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

MMP14, Membrane-type-1 matrix metalloproteinase, Membrane-type matrix metalloproteinase 1, Matrix metalloproteinase-14, Matrix metalloproteinase 14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14552-1-PBS targets MMP14 / MT1-MMP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MMP14 / MT1-MMP fusion protein Ag5965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 14 (membrane-inserted)
别名 MMP14, Membrane-type-1 matrix metalloproteinase, Membrane-type matrix metalloproteinase 1, Matrix metalloproteinase-14, Matrix metalloproteinase 14
计算分子量 66 kDa
观测分子量 66 kDa, 45-50 kDa
GenBank蛋白编号BC064803
基因名称 MMP14
Gene ID (NCBI) 4323
RRIDAB_2250751
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50281
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).