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验证数据展示

  • WB analysis using 30615-1-AP (same clone as 30615-1-PBS)

    WB analysis using 30615-1-AP (same clone as 30615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30615-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 30615-1-AP (same clone as 30615-1-PBS)

    IF Staining of A549 using 30615-1-AP (same clone as 30615-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30615-1-PBS in a different storage buffer formulation.

MMP11 Polyclonal antibody, PBS Only

MMP11 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30615-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30615-1-PBS targets MMP11 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31191

Product name: Recombinant human MMP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-208 aa of NM_005940

Sequence: VLSGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 11 (stromelysin 3)
别名
计算分子量 55 kDa
观测分子量50-55 kDa
GenBank蛋白编号NM_005940
基因名称 MMP11
Gene ID (NCBI) 4320
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24347
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

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