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验证数据展示

  • WB analysis using 11996-1-AP (same clone as 11996-1-PBS)

    WB analysis using 11996-1-AP (same clone as 11996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 11996-1-AP (same clone as 11996-1-PBS)

    WB analysis of HEK-293 using 11996-1-AP (same clone as 11996-1-PBS)

    WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 11996-1-AP (same clone as 11996-1-PBS)

    IF Staining of NIH/3T3 using 11996-1-AP (same clone as 11996-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MLK3 Polyclonal antibody, PBS Only

MLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11996-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11996-1-PBS targets MLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2636

Product name: Recombinant human MAP3K11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-379 aa of BC011263

Sequence: MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 11
别名
计算分子量 847 aa, 93 kDa
观测分子量93 and 65 kDa
GenBank蛋白编号BC011263
基因名称 MLK3
Gene ID (NCBI) 4296
RRIDAB_10694137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16584
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).

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