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验证数据展示

  • WB analysis using 31561-1-AP

    WB analysis using 31561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31561-1-AP (MIC10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 31561-1-AP

    IF Staining of HepG2 using 31561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MIC10 antibody (31561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MIC10 Polyclonal antibody

MIC10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31561-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

C1orf151, FLJ36999, FLJ75158, UPF0327 protein C1orf151

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, L02 cells, mouse heart tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31561-1-AP targets MIC10 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 MIC10 fusion protein Ag34301 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 151
别名 C1orf151, FLJ36999, FLJ75158, UPF0327 protein C1orf151
计算分子量 9 kDa
观测分子量10 kDa
GenBank蛋白编号NM_001032363
基因名称 C1orf151
Gene ID (NCBI) 440574
RRIDAB_3670036
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5TGZ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIC10, also known as C1orf151, MICOS10, and MINOS1, belongs to the  MICOS complex subunit Mic10 family. MIC10 is a component of the MICOS complex, which is a large protein complex located in the inner membrane of mitochondria. MIC10 plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. MIC10 is also functionally connected to the F1Fo-ATP synthase (PMID: 22114354, PMID: 28315355).

实验方案

Product Specific Protocols
WB protocol for MIC10 antibody 31561-1-APDownload protocol
IF protocol for MIC10 antibody 31561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols