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验证数据展示

  • WB analysis of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    WB analysis of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

    IP experiment of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

    IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    IF Staining of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

MGAT5B Polyclonal antibody, PBS Only

MGAT5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16993-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16993-1-PBS targets MGAT5B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10678

Product name: Recombinant human MGAT5B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-394 aa of BC062354

Sequence: MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGVLRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILRHSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQRQRRLRSSQEQARCGVMGACEKMLLEGDVASNTLLIEKPSKKETEKCPKNLVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
别名
计算分子量 394 aa, 44 kDa, 89 kDa
观测分子量 89-100 kDa
GenBank蛋白编号BC062354
基因名称 MGAT5B
Gene ID (NCBI) 146664
RRIDAB_10858919
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3V5L5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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