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验证数据展示

  • WB analysis using RMX00070

    WB analysis using RMX00070

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00070 (METTL5 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using RMX00070

    IHC staining of mouse kidney using RMX00070

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00070 (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL5 antibody included
  • IF Staining of HepG2 using RMX00070

    IF Staining of HepG2 using RMX00070

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (RMX00070) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Multi-rAb® METTL5 Multi-Recombinant antibody

METTL5 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00070

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRamos cells, THP-1 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy METTL5 Ready-To-Use IHC Kit. METTL5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00070 targets METTL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 methyltransferase like 5
别名 EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5
计算分子量 209 aa, 24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC000921
基因名称 METTL5
Gene ID (NCBI) 29081
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDQ9NRN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.

实验方案

Product Specific Protocols
IF protocol for METTL5 antibody RMX00070Download protocol
IHC protocol for METTL5 antibody RMX00070Download protocol
WB protocol for METTL5 antibody RMX00070Download protocol
Standard Protocols
Click here to view our Standard Protocols
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