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验证数据展示

  • WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • IP experiment of A2780 using 14994-1-AP (same clone as 14994-1-PBS)

    IP experiment of A2780 using 14994-1-AP (same clone as 14994-1-PBS)

    IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14994-1-AP (same clone as 14994-1-PBS)

    IF Staining of HepG2 using 14994-1-AP (same clone as 14994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14994-1-AP (same clone as 14994-1-PBS)

    IHC staining of human liver cancer using 14994-1-AP (same clone as 14994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14994-1-AP (METTL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL1 antibody included
  • Featured Product
  • KD/KO验证

METTL1 Polyclonal antibody, PBS Only

METTL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14994-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy METTL1 Ready-To-Use IHC Kit. METTL1 primary antibody included.

产品信息

14994-1-PBS targets METTL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6980

Product name: Recombinant human METTL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-276 aa of BC000550

Sequence: MAAETRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSLPGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 1
别名
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC000550
基因名称 METTL1
Gene ID (NCBI) 4234
RRIDAB_2142013
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBP6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

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