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METT5D1 Polyclonal antibody

METT5D1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29900-1-AP

Print datasheet

Synonyms

FLJ33979, METT5D1, METTL15

验证数据展示

  • WB analysis of rat brain using 29900-1-AP

    WB analysis of rat brain using 29900-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29900-1-AP

    WB analysis using 29900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29900-1-AP (METT5D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 29900-1-AP

    IHC staining of mouse colon using 29900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29900-1-AP (METT5D1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 29900-1-AP

    IHC staining of mouse colon using 29900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29900-1-AP (METT5D1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, THP-1 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29900-1-AP targets METT5D1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen METT5D1 fusion protein Ag31863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase 5 domain containing 1
Synonyms FLJ33979, METT5D1, METTL15
Observed Molecular Weight37-45 kDa
GenBank Accession NumberBC030997
Gene Symbol METT5D1
Gene ID (NCBI) 196074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NJ78
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for METT5D1 antibody 29900-1-APDownload protocol
IHC protocol for METT5D1 antibody 29900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols