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验证数据展示

  • WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

    WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

    IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

    IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

    IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

    IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MBD3 Polyclonal antibody, PBS Only

MBD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14258-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14258-1-PBS targets MBD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MBD3 fusion protein Ag5531 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyl-CpG binding domain protein 3
别名
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC043619
基因名称 MBD3
Gene ID (NCBI) 53615
RRIDAB_2139745
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95983
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .