验证数据展示
经过测试的应用
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32526-1-AP targets MAdCAM1 in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2010 Product name: Recombinant Mouse MAdCAM-1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 22-364 aa of NP_038619 Sequence: QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGQVTNLYVPGQVTPNSSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mucosal vascular addressin cell adhesion molecule 1 |
| 别名 | Adhesion molecule immunoglobulin-like domain-containing protein, MAdCAM-1 |
| 计算分子量 | 44kDa |
| GenBank蛋白编号 | NP_038619 |
| 基因名称 | Madcam1 |
| Gene ID (NCBI) | 17123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MAdCAM1 antibody 32526-1-AP | Download protocol |
| IHC protocol for MAdCAM1 antibody 32526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
