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验证数据展示

  • IHC staining of human testis using 84248-8-RR

    IHC staining of human testis using 84248-8-RR

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 84248-8-RR (MAGEA4 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 84248-8-RR

    IHC staining of human testis using 84248-8-RR

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 84248-8-RR (MAGEA4 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAGEA4 Recombinant monoclonal antibody

MAGEA4 Uni-rAb® Recombinant Antibody for IHC, ELISA
Cat No. 84248-8-RR

产品说明书

CloneNo. 241558D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84248-8-RR targets MAGEA4 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3193

Product name: Recombinant human MAGEA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC017723

Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen
计算分子量 317 aa, 35 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP43358
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members, which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021)

实验方案

Product Specific Protocols
IHC protocol for MAGEA4 antibody 84248-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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