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验证数据展示

  • WB analysis using 29663-1-AP

    WB analysis using 29663-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29663-1-AP (MADD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 29663-1-AP

    IHC staining of human ovary cancer using 29663-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29663-1-AP (MADD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MADD antibody included

MADD Polyclonal antibody

MADD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29663-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MAP kinase-activating death domain protein, KIAA0358, IG20, FLJ36300, FLJ35600

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MADD Ready-To-Use IHC Kit. MADD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29663-1-AP targets MADD in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31292

Product name: Recombinant human MADD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1395-1544 aa of BC040484

Sequence: WRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP-kinase activating death domain
别名 MAP kinase-activating death domain protein, KIAA0358, IG20, FLJ36300, FLJ35600
计算分子量 1544 aa, 171 kDa
观测分子量200-250 kDa
GenBank蛋白编号BC040484
基因名称 MADD
Gene ID (NCBI) 8567
RRIDAB_3086151
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXG6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MADD, MAP kinase-activating death domain protein, belongs to the DENN family, which regulates the Rab family is small GTPases (PMID: 20937701, PMID: 18559336). MADD plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. MADD is also involved in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PMID: 32761064). KIF1Bβ- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through MADD (PMID: 18849981).

实验方案

Product Specific Protocols
IHC protocol for MADD antibody 29663-1-APDownload protocol
WB protocol for MADD antibody 29663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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