ELISA experiment of PEG using 87604-1-RR

96-well plates were coated with Peptied PEG12, mPEG20K-Biotin, Biotin-PEG2K-Biotin. The plate was blocked with 1% BSA blocking buffer, then washed, and incubated with 100 µL per well of PEG antibody 87604-1-RR and serially diluted with a starting concentration of 2 µg/mL (1 hr, 37℃). The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:10,000 (1 hr, 37℃). Detection was performed using an Ultra TMB Substrate (3-5 min, room temp). The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.