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验证数据展示

  • WB analysis using 32754-1-AP

    WB analysis using 32754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 32754-1-AP

    WB analysis using 32754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 32754-1-AP

    IHC staining of human tonsillitis using 32754-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LY75/DEC-205 Polyclonal antibody

LY75/DEC-205 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32754-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CD 205, LY75, CD205, DEC 205, DEC-205

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, mouse thymus tissue, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32754-1-AP targets LY75/DEC-205 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LY75/DEC-205 fusion protein Eg3439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 75
别名 CD 205, LY75, CD205, DEC 205, DEC-205
计算分子量198kDa
观测分子量198-250 kDa
GenBank蛋白编号NM_002349.4
基因名称 CD205
Gene ID (NCBI) 4065
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60449-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY75, also known as DEC-205 or CD205, is a type I transmembrane protein belonging to the C-type lectin receptor family. It is primarily expressed on dendritic cells (DCs) and thymic epithelium, but is also found on monocytes, T and B lymphocytes, and various other tissues, including intestinal and pulmonary epithelia.

实验方案

Product Specific Protocols
WB protocol for LY75/DEC-205 antibody 32754-1-APDownload protocol
IHC protocol for LY75/DEC-205 antibody 32754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols