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验证数据展示

  • WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18693-1-AP (LSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    IP result of anti-LSS (IP:18693-1-AP, 4ug; Detection:18693-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

    IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18693-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

LSS Polyclonal antibody, PBS Only

LSS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18693-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18693-1-PBS targets LSS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6955

Product name: Recombinant human LSS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 382-732 aa of BC035638

Sequence: NGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYVQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRFSQLYPERALAGHP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
别名
计算分子量 732 aa, 83 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC035638
基因名称 LSS
Gene ID (NCBI) 4047
RRIDAB_2138608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48449
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

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