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验证数据展示

  • IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 22899-1-AP (same clone as 22899-1-PBS)

    IHC staining of human lung cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • WB analysis using 22899-1-AP (same clone as 22899-1-PBS)

    WB analysis using 22899-1-AP (same clone as 22899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22899-1-AP (LMTK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

LMTK3 Polyclonal antibody, PBS Only

LMTK3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22899-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

22899-1-PBS targets LMTK3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lemur tyrosine kinase 3
别名
计算分子量 154 kDa
观测分子量~155 kDa
GenBank蛋白编号NM_001080434
基因名称 LMTK3
Gene ID (NCBI) 114783
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96Q04
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LMTK3 is enriched at a perinuclear structure around Golgi membranes, it is present as a dotted pattern throughout neurites, and localized at neuronal synapses. It is also expected to be expressed in brain and duodenum. LMTK3 is a serine/threonine protein kinase expressed predominantly in brain. LMTK3 regulated ER-alpha stability and activity directly at the mRNA level via downregulation of PKC catalytic activity, resulting in less phosphorylated AKT at ser473. In vitro kinase assays indicated that LMTK3 inhibited the ability of PKC to phosphorylate ER-alpha, thereby affecting ER-alpha activity indirectly at the protein level by protecting it from proteasomal degradation. In agreement, analysis with a breast tumor xenograft mouse model revealed that knockdown of LMTK3 reduced proliferation of ER-alpha-positive breast cancer cells and reduced tumor growth. In addition, LMTK3 abundance and intronic polymorphisms were significantly associated with human cancers, overall patient survival, and predicted response to endocrine therapies. The calculated molecular weight of LMTK3 is 153 kDa.