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验证数据展示

  • WB analysis using 26561-1-AP

    WB analysis using 26561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26561-1-AP (LIMCH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 26561-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26561-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26561-1-AP (LIMCH1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LIMCH1 antibody included

LIMCH1 Polyclonal antibody

LIMCH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26561-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

LMO7B, LIMCH1A, LIM and calponin homology domains-containing protein 1, KIAA1102

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LIMCH1 Ready-To-Use IHC Kit. LIMCH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26561-1-AP targets LIMCH1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24112

Product name: Recombinant human LIMCH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-566 aa of BC023546

Sequence: RWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMSGKVELVLSQKVVKPKSPEPEATL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM and calponin homology domains 1
别名 LMO7B, LIMCH1A, LIM and calponin homology domains-containing protein 1, KIAA1102
观测分子量~150 kDa
GenBank蛋白编号BC023546
基因名称 LIMCH1
Gene ID (NCBI) 22998
RRIDAB_3085883
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIM and calponin-homology domains 1 (LIMCH1) was identified from a border cell migration screen based on conditional ectopic expression in Drosophila. It's a kind of actin stress fibers-associated protein that activates non-muscle myosin IIa. Through the activation of non-muscle myosin IIa, LIMCH1 positively regulates actin stress fibers assembly and stabilizes focal adhesions, negatively regulates cell spreading and cell migration.

实验方案

Product Specific Protocols
WB protocol for LIMCH1 antibody 26561-1-APDownload protocol
IHC protocol for LIMCH1 antibody 26561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols