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验证数据展示

  • WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

    WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LIG4 Polyclonal antibody, PBS Only

LIG4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12695-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12695-1-PBS targets LIG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LIG4 fusion protein Ag3385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ligase IV, DNA, ATP-dependent
别名
计算分子量 911 aa, 104 kDa
观测分子量 100-104 kDa
GenBank蛋白编号BC037491
基因名称 LIG4
Gene ID (NCBI) 3981
RRIDAB_2136253
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49917
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.