验证数据展示

WB analysis of human skeletal muscle using 22779-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 22779-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 22779-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 22779-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 22779-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 22779-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22779-1-AP (LIFR Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 22779-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22779-1-AP (LIFR Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 22779-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIFR antibody (22779-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IP experiment of mouse skeletal muscle using 22779-1-AP
IP result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.
IF Staining of mouse cerebellum using 22779-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LIFR antibody (22779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse cerebellum using 22779-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LIFR antibody (22779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

LIFR Polyclonal antibody

LIFR Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 22779-1-AP

产品说明书

引用文献(20)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

CD118, LIF R, LIF receptor, LIF-R, SJS2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (20)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	human skeletal muscle tissue, HeLa cells, human placenta tissue, mouse skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human skeletal muscle tissue, human kidney tissue, human placenta tissue, mouse cerebellum tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue
Positive IF/ICC detected in	HeLa cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 4 publications below
IF	See 5 publications below

产品信息

22779-1-AP targets LIFR in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, pig
免疫原	CatNo: Ag18742 Product name: Recombinant human LIFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 906-1097 aa of BC153096 Sequence: NNVEVLETRSAFPKIEDTEIISPVAERPEDRSDAEPENHVVVSYCPPIIEEEIPNPAADEAGGTAQVIYIDVQSMYQPQAKPEEEQENDPVGGAGYKPQMHLPINSTVEDIAAEEDLDKTAGYRPQANVNTWNLVSPDSPRSIDSNSEIVSFGSPCSINSRQFLIPPKDEDSPKSNGGGWSFTNFFQNKPND 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	leukemia inhibitory factor receptor alpha
别名	CD118, LIF R, LIF receptor, LIF-R, SJS2
计算分子量	1097 aa, 124 kDa
观测分子量	190 kDa, 170 kDa
GenBank蛋白编号	BC153096
基因名称	LIFR
Gene ID (NCBI)	3977
RRID	AB_2879165
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen Affinity purified
UNIPROT ID	P42702
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183). LIFR appeared as doublets with 190 and 170 kDa (PMID: 10858440). They represented different glycosylated forms of the LIFR in different steps of protein maturation.

实验方案

Product Specific Protocols
IF protocol for LIFR antibody 22779-1-AP	Download protocol
IHC protocol for LIFR antibody 22779-1-AP	Download protocol
IP protocol for LIFR antibody 22779-1-AP	Download protocol
WB protocol for LIFR antibody 22779-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse		Nature Exercise plasma boosts memory and dampens brain inflammation via clusterin. Authors - Zurine De Miguel View Article
mouse	WB,IF	Nat Metab LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration Authors - Yalan Deng View Article
mouse	WB	Nat Commun A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis. Authors - Fan Yao KO Validated View Article
human	WB	Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. Authors - An-Dong Liu View Article
human	WB,IHC,IF	Hum Mol Genet Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations. Authors - Anne Kosfeld View Article
human	WB	J Exp Clin Cancer Res miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop. Authors - Feifei Zhang View Article

验证数据展示

WB analysis of human skeletal muscle using 22779-1-AP

WB analysis using 22779-1-AP

WB analysis using 22779-1-AP

IHC staining of mouse skeletal muscle using 22779-1-AP

IHC staining of mouse cerebellum using 22779-1-AP

IHC staining of human skeletal muscle using 22779-1-AP

IHC staining of human skeletal muscle using 22779-1-AP

IF Staining of HeLa using 22779-1-AP

IP experiment of mouse skeletal muscle using 22779-1-AP

IF Staining of mouse cerebellum using 22779-1-AP

IF Staining of mouse cerebellum using 22779-1-AP

LIFR Polyclonal antibody

LIFR Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Exercise plasma boosts memory and dampens brain inflammation via clusterin.

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations.

miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop.