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验证数据展示

  • WB analysis using 30409-1-AP

    WB analysis using 30409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30409-1-AP (LGALS9/Galectin-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

LGALS9/Galectin-9 Polyclonal antibody

LGALS9/Galectin-9 Polyclonal Antibody for WB, ELISA
Cat No. 30409-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS9 Ready-To-Use IHC Kit. LGALS9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30409-1-AP targets LGALS9/Galectin-9 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 LGALS9/Galectin-9 fusion protein Eg0084 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 9
别名 Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9
计算分子量 323 aa, 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC110340
基因名称 Galectin-9
Gene ID (NCBI) 3965
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00182
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

实验方案

Product Specific Protocols
WB protocol for LGALS9/Galectin-9 antibody 30409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols