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验证数据展示

  • WB analysis using 32958-1-AP

    WB analysis using 32958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32958-1-AP (LAMTOR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 32958-1-AP

    IP experiment of HeLa using 32958-1-AP

    IP result of anti-LAMTOR4 (IP:32958-1-AP, 4ug; Detection:32958-1-AP 1:1000) with HeLa cells lysate 1600 ug.

LAMTOR4 Polyclonal antibody

LAMTOR4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32958-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

C7orf59, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4, Ragulator complex protein LAMTOR4, Ragulator complex protein LAMTOR4, N-terminally processed

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, PANC-1 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32958-1-AP targets LAMTOR4 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag39105

Product name: Recombinant human C7orf59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC130553

Sequence: MTSALTQGLERIPDQLGYLVLSEGAVLASSGDLENDEQAASAISELVSTACGFRLHRGMNVPFKRLSVVFGEHTLLVTVSGQRVFVVKRQNRGREPIDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 59
别名 C7orf59, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4, Ragulator complex protein LAMTOR4, Ragulator complex protein LAMTOR4, N-terminally processed
计算分子量11 kDa
观测分子量11 kDa
GenBank蛋白编号BC130553
基因名称 C7orf59
Gene ID (NCBI) 389541
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ0VGL1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 (LAMTOR4) is a protein belonging to the LAMTOR family and believed to be involved in cell cycle, proliferation, and growth (PMID: 29138253). LAMTOR4 has been reported as a regulator complex that acts as an activator of rapamycin complex 1 (mTORC1) by sensing changes in the levels of amino acids (PMID: 33429026). It might offer a strategy to inhibit tumor progression and metastasis in prostate cancer (PMID: 39125671).

实验方案

Product Specific Protocols
IP protocol for LAMTOR4 antibody 32958-1-APDownload protocol
WB protocol for LAMTOR4 antibody 32958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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