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验证数据展示

  • WB analysis using 22215-1-AP (same clone as 22215-1-PBS)

    WB analysis using 22215-1-AP (same clone as 22215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22215-1-AP (Kindlin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

    IF Staining of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FERMT1/Kindlin 1 antibody included
  • IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FERMT1/Kindlin 1 antibody included
  • Featured Product
  • KD/KO验证

Kindlin 1 Polyclonal antibody, PBS Only

Kindlin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22215-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FERMT1/Kindlin 1 Ready-To-Use IHC Kit. FERMT1/Kindlin 1 primary antibody included.

产品信息

22215-1-PBS targets Kindlin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17543

Product name: Recombinant human Kindlin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 321-420 aa of BC035882

Sequence: HISKLSLSAETQDFAGESEVDEIEAALSNLEVTLEGGKADSLLEDITDIPKLADNLKLFRPKKLLPKAFKQYWFIFKDTSIAYFKNKELEQGEPLEKLNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fermitin family homolog 1 (Drosophila)
别名
计算分子量 677 aa, 77 kDa
观测分子量 70-77 kDa
GenBank蛋白编号BC035882
基因名称 Kindlin 1
Gene ID (NCBI) 55612
RRIDAB_2879033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQL6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).

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