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验证数据展示

  • IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (21017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KLF5 Polyclonal antibody, PBS Only

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21017-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

21017-1-PBS targets KLF5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15267

Product name: Recombinant human KLF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC042131

Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kruppel-like factor 5 (intestinal)
别名
计算分子量 457 aa, 51 kDa
观测分子量 51-55 kDa
GenBank蛋白编号BC042131
基因名称 KLF5
Gene ID (NCBI) 688
RRIDAB_10696447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13887
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

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