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验证数据展示

  • WB analysis using 25468-1-AP

    WB analysis using 25468-1-AP

    Rat brain tissues (heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 25468-1-AP (KCND3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 25468-1-AP

    IHC staining of mouse cerebellum using 25468-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25468-1-AP

    IHC staining of mouse brain using 25468-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCND3 Polyclonal antibody

KCND3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25468-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KV4.3, KSHIVB, KCND3S, KCND3L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, 37℃ incubated rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25468-1-AP targets KCND3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22183

Product name: Recombinant human KCND3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 602-636 aa of BC113477

Sequence: PALTPEGESRPPPASPGPNTNIPSIASNVVKVSAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, Shal-related subfamily, member 3
别名 KV4.3, KSHIVB, KCND3S, KCND3L
计算分子量 655 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC113477
基因名称 KCND3
Gene ID (NCBI) 3752
RRIDAB_3669478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCND3 gene encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 gene defect causes both cardiological and neurological syndromes. Kv4.3 contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential.

实验方案

Product Specific Protocols
WB protocol for KCND3 antibody 25468-1-APDownload protocol
IHC protocol for KCND3 antibody 25468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols