KC7F2

HIF1α:20 μM

KC7F2在戊四氮癫痫模型大鼠中显著缩短潜伏期，并在慢性阶段增加了锂-毛果芸香碱大鼠模型中自发性复发性癫痫的发作率。[3]

KC7F2在LN229-HRE-AP细胞中抑制HRE驱动的转录并降低HIF-1α蛋白水平。对于MCF7、LNZ308、A549、U251 mg和LN229癌细胞，KC7F2表现出剂量响应性细胞毒性，IC50约为15至25μM。在D54 mg胶质瘤细胞中，尤其在缺氧条件下，KC7F2抑制了集落形成。[1] 在缺氧的小胶质细胞培养中，KC7F2下调TfR和DMT的表达，并减少了HIF-1α介导的铁积累。[2]

DMSO:57 mg/mL (99.93 mM)

Cells are seeded onto 96-well plates (4 × 103/well) and cultured under normoxic (21% O2) and hypoxic (1% O2) conditions with different concentrations of KC7F2 for 72 h or treated for various times with 20 μM KC7F2. For proliferation analysis, cells are fixed with 50% trichloroacetic acid for 1 h at 4°C, followed by staining with 0.4% sulforhodamine B dissolved in 1% acetic acid for 30 min at room temperature. Plates are washed five times with 1% acetic acid to remove unbound dye. Bound dye is dissolved by adding 10 mM unbuffered Tris base. Cell proliferation is calculated by measuring OD values at 564 nm using a spectrophotometer.(Only for Reference)