验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, RKO cells, U-118 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31970-1-AP targets KBTBD8 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36219 Product name: Recombinant human KBTBD8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-420 aa of NM_032505.2 Sequence: QELGDRSKEYIRKKFLCVTKEQEFLQLTKDQLISILDSDDLNVDREEHVYESIIRWFEHEQNEREVHLPEIFAKCIRFPLMEDTFIEKIPPQFAQAIAKSCVEKGPSNTNGCTQRLGMTASEMIICFDAAHKHSGKKQTVPCLDIVTGRVFKLCKPPNDLREVGILVSPDNDIYIAGGYRPSSSEVSIDHKAENDFWMYDHSTNRWLSKPSLLRARIGCKLVYCCGKMYAIGGRVYEGDGRNSLKSVECYDSRENC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kelch repeat and BTB (POZ) domain containing 8 |
| 别名 | T-cell activation kelch repeat protein, TA-KRP, TAKRP, KIAA1842, Kelch repeat and BTB domain-containing protein 8 |
| 计算分子量 | 69 kDa，601aa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_032505.2 |
| 基因名称 | KBTBD8 |
| Gene ID (NCBI) | 84541 |
| RRID | AB_3670161 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8NFY9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kelch repeat and BTB domain containing 8 (KBTBD8, also known as TAKRP and TA-KRP) is a member of the BTB-kelch family of proteins, which is specifically localized at the Golgi apparatus in nondividing cells and becomes restricted to the spindle apparatus upon mitosis (PMID: 23578279). KBTBD family proteins are involved in ubiquitination in various cells and tissues and are correlated with human muscle diseases (PMID: 9878064; 16547521; 24959344). The expression level of KBTBD8 might affect the proliferation and migration of epithelial ovarian cancer (PMID: 33109073).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KBTBD8 antibody 31970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
