JNK-IN-8

CAS号

1410880-22-6

分子式

C29H29N7O2

主要靶点

c-Kit|JNK

仅限科研使用

Cat No : CM04926

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Synonyms

Inhibitor|inhibit|Kit (V559D)|Kit (V559D,T670I)|JNKIN8|JNK-IN-8|JNK|JNK IN 8|JNK Inhibitor XVI|JNK1|JNK2|JNK3|cKit



产品信息

CAS号 1410880-22-6
分子式 C29H29N7O2
主要靶点 c-Kit|JNK
主要通路 蛋白酪氨酸激酶|MAPK 信号通路
分子量 507.59
纯度 100%, 此纯度可做参考,具体纯度与批次有关系,可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Inhibitor|inhibit|Kit (V559D)|Kit (V559D,T670I)|JNKIN8|JNK-IN-8|JNK|JNK IN 8|JNK Inhibitor XVI|JNK1|JNK2|JNK3|cKit

靶点活性

JNK2:18.7 nM|JNK1:4.7 nM|JNK:1 nM

体内活性

JNK-IN-8(10 mM)对IL-1R细胞中IL-1β刺激的c-Jun磷酸化有抑制作用。与伊马替尼相比,JNK-IN-8有明显的1,4-双苯胺和1,3-氨基苯甲酸结构区域选择性,且以N,N-二甲基丁烯乙酰胺共价结合Cys154靶点。JNK-IN-8抑制HeLa(EC50:486 nM)和A375(EC50:338 nM)细胞中c-Jun的磷酸化。JNK-IN-8与PIK3C3,IRAK1,PIP5K3和PIP4K2C结合可使选择性和消除率显著提高。JNK-IN-8经Cys116抑制JNK2。

溶解度

H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (108.36 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

细胞实验

JNK-IN-8 is dissolved in DMSO and stored, and then diluted with appropriate media before use[1]. HEK-293 cells stably expressing Interleukin Receptor 1 (HEK293-IL1R) are cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM glutamine and 1×antimycotic/antibiotic solution. Cells are serum starved for 18 h before incubation with DMSO or JNK-IN-8, stimulated with 2 μM Anisomycin for 1h and lysates are clarified by centrifugation for 10 min at 16000 g and 4°C[1].

参考文献

1.Zhang T, et al. Chem Biol, 2012, 19(1), 140-154.
2.Goh ET, et al. Biochem J, 2012, 441(1), 339-346.
3.Liu Q, et al. Chem Biol, 2013, 20(2), 146-159.

The molarity calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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