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IPMK Polyclonal antibody

IPMK Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30161-1-AP

Print datasheet

Synonyms

IMPK, IPMK

验证数据展示

  • WB analysis using 30161-1-AP

    WB analysis using 30161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30161-1-AP (IPMK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30161-1-AP

    IF Staining of A431 using 30161-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IPMK antibody (30161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30161-1-AP targets IPMK in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen IPMK fusion protein Ag32377 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate multikinase
Synonyms IMPK, IPMK
Observed Molecular Weight42-47 kDa
GenBank Accession NumberBC065709
Gene Symbol IPMK
Gene ID (NCBI) 253430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).

实验方案

Product Specific Protocols
WB protocol for IPMK antibody 30161-1-APDownload protocol
IF protocol for IPMK antibody 30161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols