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验证数据展示

  • WB analysis using 30161-1-AP

    WB analysis using 30161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30161-1-AP (IPMK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30161-1-AP

    IF Staining of A431 using 30161-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IPMK antibody (30161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

IPMK Polyclonal antibody

IPMK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30161-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

IMPK, IPMK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy IPMK Ready-To-Use IHC Kit. IPMK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30161-1-AP targets IPMK in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 IPMK fusion protein Ag32377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate multikinase
别名 IMPK, IPMK
观测分子量42-47 kDa
GenBank蛋白编号BC065709
基因名称 IPMK
Gene ID (NCBI) 253430
RRIDAB_3086252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).

实验方案

Product Specific Protocols
WB protocol for IPMK antibody 30161-1-APDownload protocol
IF protocol for IPMK antibody 30161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols