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验证数据展示

  • WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

    WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

    WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 55282-1-AP (same clone as 55282-1-PBS)

    IF Staining of HEK-293 using 55282-1-AP (same clone as 55282-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

INSIG1 Polyclonal antibody, PBS Only

INSIG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55282-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

55282-1-PBS targets INSIG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin induced gene 1
别名 CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein
计算分子量 30 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_005542
基因名称 INSIG1
Gene ID (NCBI) 3638
RRIDAB_2881298
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15503
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

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