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验证数据展示

  • WB analysis using 27240-1-AP

    WB analysis using 27240-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 27240-1-AP

    IP experiment of MCF-7 using 27240-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:500.

INPP5F Polyclonal antibody

INPP5F Polyclonal Antibody for WB, IP, ELISA
Cat No. 27240-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

hSAC2, INPP5F, KIAA0966, MSTP007, MSTP047, MSTPO47, SAC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27240-1-AP targets INPP5F in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 INPP5F fusion protein Ag25687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase F
别名 hSAC2, INPP5F, KIAA0966, MSTP007, MSTP047, MSTPO47, SAC2
计算分子量 1132 aa, 128 kDa
观测分子量128-140 kDa
GenBank蛋白编号BC111493
基因名称 INPP5F
Gene ID (NCBI) 22876
RRIDAB_3095738
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2H2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5F can negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus.

实验方案

Product Specific Protocols
WB protocol for INPP5F antibody 27240-1-APDownload protocol
IP protocol for INPP5F antibody 27240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols