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验证数据展示

  • WB analysis using 15890-1-AP

    WB analysis using 15890-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15890-1-AP (INPP4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15890-1-AP

    IHC staining of human lung cancer using 15890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15890-1-AP (INPP4B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15890-1-AP

    IHC staining of human lung cancer using 15890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15890-1-AP (INPP4B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INPP4B Polyclonal antibody

INPP4B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15890-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

INPP4B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15890-1-AP targets INPP4B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8676

Product name: Recombinant human INPP4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-53 aa of BC005273

Sequence: MEIKEEGASEEGQHFLPTAQANDPGDCQFTSIQKTPNEPQLEFILDLNQELRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-4-phosphatase, type II, 105kDa
别名 INPP4B
计算分子量 53 aa, 6 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC005273
基因名称 INPP4B
Gene ID (NCBI) 8821
RRIDAB_3669224
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

实验方案

Product Specific Protocols
IHC protocol for INPP4B antibody 15890-1-APDownload protocol
WB protocol for INPP4B antibody 15890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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