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验证数据展示

  • WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

    IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING5 antibody included
  • IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 10665-1-AP (same clone as 10665-1-PBS)

    IHC staining of human thyroid cancer using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING5 antibody included
  • IHC staining of rat skin using 10665-1-AP (same clone as 10665-1-PBS)

    IHC staining of rat skin using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING5 antibody included
  • Featured Product
  • KD/KO验证

ING5 Polyclonal antibody, PBS Only

ING5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10665-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Inhibitor of growth protein 5, p28ING5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ING5 Ready-To-Use IHC Kit. ING5 primary antibody included.

产品信息

10665-1-PBS targets ING5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1059

Product name: Recombinant human ING5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC005370

Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inhibitor of growth family, member 5
别名 Inhibitor of growth protein 5, p28ING5
计算分子量 28 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC005370
基因名称 ING5
Gene ID (NCBI) 84289
RRIDAB_2280522
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYH8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

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