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验证数据展示

  • WB analysis using 84532-4-RR

    WB analysis using 84532-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84532-4-RR (IL-1RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84532-4-RR

    Affinity and Kinetic Characterization of 84532-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84532-4-RR against Human IL-1RA were performed. The affinity constant is 0.302 nM.

IL-1RA Recombinant antibody

IL-1RA Recombinant Antibody for WB, ELISA
Cat No. 84532-4-RR

产品说明书

CloneNo. 241879B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

IL 1ra, IL1RN, IL1RA, IL 1RN, ICIL-1RA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84532-4-RR targets IL-1RA in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 IL-1RA fusion protein Ag1277 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1 receptor antagonist
别名 IL 1ra, IL1RN, IL1RA, IL 1RN, ICIL-1RA
计算分子量 20 kDa
观测分子量16-18 kDa
GenBank蛋白编号BC009745
基因名称 IL-1RA
Gene ID (NCBI) 3557
RRIDAB_3672040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP18510
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

实验方案

Product Specific Protocols
WB protocol for IL-1RA antibody 84532-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols