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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66460-1-Ig (same clone as 66460-1-PBS)

    WB analysis using 66460-1-Ig (same clone as 66460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66460-1-Ig (IKBKG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKBKG antibody included
  • IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKBKG antibody included
  • IF Staining of HeLa using 66460-1-Ig (same clone as 66460-1-PBS)

    IF Staining of HeLa using 66460-1-Ig (same clone as 66460-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IKBKG antibody (66460-1-Ig, Clone: 1F2E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

IKBKG Monoclonal antibody, PBS Only

IKBKG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66460-1-PBS

产品说明书

CloneNo. 1F2E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IKBKG Ready-To-Use IHC Kit. IKBKG primary antibody included.

产品信息

66460-1-PBS targets IKBKG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 IKBKG fusion protein Ag13358 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
别名 AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC012114
基因名称 IKBKG
Gene ID (NCBI) 8517
RRIDAB_2881829
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6K9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.