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验证数据展示

  • WB analysis using 32052-1-AP

    WB analysis using 32052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32052-1-AP (IGFBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, ELISA
Cat No. 32052-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA

别名

IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse serum

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32052-1-AP targets IGFBP2 in WB, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1196

Product name: Recombinant Mouse IGFBP2 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 35-305 aa of NM_008342.3

Sequence: EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 2
别名 IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2
计算分子量33kDa
观测分子量33 kDa
GenBank蛋白编号NM_008342.3
基因名称 Igfbp2
Gene ID (NCBI) 16008
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP47877
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 32052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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