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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 17511-1-AP (same clone as 17511-1-PBS)

    WB analysis using 17511-1-AP (same clone as 17511-1-PBS)

    Jurkat cells lysates were subjected to SDS PAGE followed by western blot with 17511-1-AP (ICMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17511-1-PBS in a different storage buffer formulation.

ICMT Polyclonal antibody, PBS Only

ICMT Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17511-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

17511-1-PBS targets ICMT in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isoprenylcysteine carboxyl methyltransferase
别名
计算分子量 32 kDa
观测分子量 32-34 kDa
GenBank蛋白编号NM_012405
基因名称 ICMT
Gene ID (NCBI) 23463
RRIDAB_2293346
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60725
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Isoprenylcysteine carboxylmethyltransferase (ICMT), also known as PCCMT, is a multi-spanning endoplasmic reticulum integral membrane protein conserved among eukaryotic organisms. Methylation by ICMT is important for partitioning of CAAX proteins into lipid membranes as this modification neutralizes the negative charge on the carboxylate and increases the hydrophobicity of the modified protein (PMID: 25059662). Homozygous gene knockout of PPMT interferes with localization of K-Ras to the plasma membrane in mammalian cells and leads to the death of mouse embryos during gestation (PMID: 12141948).