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Hygromycin B

CAS号

31282-04-9

分子式

C20H37N3O13

主要靶点

Antibiotic|Antibacterial|Antifungal|ribosome

仅限科研使用

Cat No : CM00298

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Synonyms

Inhibitor|潮霉素B|inhibit|Hygromycin B|Hygrovetine|Fungal|Bacterial|70S ribosome|Antibiotic

验证数据展示

  • CAS No.: 31282-04-9

    Hygromycin B
    CAS#: 31282-04-9

产品信息

CAS号 31282-04-9
分子式 C20H37N3O13
主要靶点 Antibiotic|Antibacterial|Antifungal|ribosome
主要通路 微生物学|微生物学|微生物学|微生物学
分子量 527.52
纯度 99.9%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Inhibitor|潮霉素B|inhibit|Hygromycin B|Hygrovetine|Fungal|Bacterial|70S ribosome|Antibiotic

体内活性

方法：为检测体内抗病毒活性，将 Hygromycin B (0-5 μmoL/kg) 腹腔注射给 MHV-A59 感染的 BALB/c 小鼠，每天两次，持续三天。 结果：Hygromycin B 能够在体内降低病毒复制和坏死肝灶的水平。[3]

体外活性

方法：小鼠 PDAC 细胞 14387T 用 lentiCRISPRv2 hygro 慢病毒转染细胞，48 h 后，将转染的细胞在含有 Hygromycin B (500μg/mL) 的新培养基中培养两周，筛选转染成功的细胞。 结果：Puromycin dihydrochloride 筛选出了慢病毒成功转染的细胞。[1] 方法：大肠杆菌用含 Hygromycin B (0-8 μg/mL) 的培养基培养，使用 Klett-Summerson colorimeter 监测细胞生长。 结果：Hygromycin B 以浓度依赖的方式减少了活细胞的数量并增加了倍增时间。Hygromycin B 在 20 μg/mL 浓度下抑制 50% 的活细胞数，在 25 μg/mL 浓度时生长速率减半。[2]

溶解度

DMSO:30 mg/mL (56.87 mM); Saline:50 mg/mL (94.78 mM)

参考文献

1.Fan M, et al. UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis. Cell Rep. 2024 Feb 16;43(2):113808.
2.McGaha SM, et al. Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli. Antimicrob Agents Chemother. 2007 Feb;51(2):591-6.
3.Macintyre G, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrob Agents Chemother. 1991 Oct;35(10):2125-7.
4.Santerre RF, et al. Gene, 1984, 30(1-3), 147-156.
5.Hanif M, et al. Curr Genet, 2002, 41(3), 183-188.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
=
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×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2