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Recombinant Human SPINT1 protein (rFc Tag) (HPLC verified)

种属

Human

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg3131

Print datasheet

Synonyms

HAI 1, HAI-1, HAI, HAI1, Kunitz-type protease inhibitor 1

验证数据展示

  • Purity of Recombinant Human SPINT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human SPINT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human SPINT1 was greater than 90% as determined by SEC-HPLC.

    The purity of Human SPINT1 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human SPINT1 protein Ala35-Glu465 (Accession# O43278-1) with a rabbit IgG Fc tag at the C-terminus.
基因ID 6692
蛋白编号 O43278-1
预测分子量 73.8 kDa
SDS-PAGE 70-80 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

SPINT1, or Serine Peptidase Inhibitor, Kunitz Type 1, is a protein encoded by the SPINT1 gene in humans. SPINT1 functions as a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. It has been shown to modulate HGF/MET activation in certain cancer types, suggesting its role in tumor biology (PMID: 29532159). Furthermore, SPINT1 has been implicated in glucose homeostasis and insulin production. Studies have shown that SPINT1 disruption in mouse pancreas leads to glucose intolerance and impaired insulin production, involving the HEPSIN/MAFA signaling pathway (PMID: 39627229).

参考文献:

1. Liu CL. et al. (2018). Histochem Cell Biol. 149(6):635-644.
2. Lin HH. et al. (2024). Nat Commun. 15(1):10537.