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验证数据展示

  • IHC staining of human tonsillitis using 98804-3-RR

    IHC staining of human tonsillitis using 98804-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98721-9-RR (Human IgG1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of human PBMCs using 98804-3-RR

    FC experiment of human PBMCs using 98804-3-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Human IgG1 Rabbit RecAb (98804-3-RR, Clone: 251296E10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Anti-Human IgG1 Rabbit Recombinant Antibody

IgG1 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98804-3-RR

产品说明书

CloneNo. 251296E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

Human IgG1, IGHG1, Immunoglobulin heavy constant gamma 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98804-3-RR targets IgG1 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg6672

Product name: recombinant human IgG1-IgK protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10009498 of

Sequence: /

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin heavy constant gamma 1 (G1m marker)
别名 Human IgG1, IGHG1, Immunoglobulin heavy constant gamma 1
基因名称 IGHG1
Gene ID (NCBI) 3500
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Human Immunoglobulin G subclass 1 (IgG1) is the most abundant antibody subclass in human serum, constituting approximately 60-70% of total IgG. It serves as a critical mediator of the adaptive immune system's defense against pathogens. Human IgG1 represents a perfect balance of specificity, stability, and powerful immune-activating capacity. Its dominance in the biotherapeutic landscape is a testament to its versatility and effectiveness. This antibody does not recognize IgA2.

实验方案

Product Specific Protocols
FC protocol for IgG1 antibody 98804-3-RRDownload protocol
IHC protocol for IgG1 antibody 98804-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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