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Recombinant Human Granzyme B protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0592

Print datasheet

Synonyms

GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1

验证数据展示

  • Purity of Recombinant Human Granzyme B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Granzyme B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 CHO-derived Human Granzyme B protein Gly19-Tyr247 (Accession# P10144) with a His tag at the C-terminus.
基因ID 3002
蛋白编号 P10144
预测分子量 27.4 kDa
SDS-PAGE 30-35 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 300 mM NaCl, pH 7.4. Normally 2.5% trehalose and 2.5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization. GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA. This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei. GzmB also forms dimers.

参考文献:

1. Cullen, Sean P et al. The Journal of cell biology vol. 176,4 (2007): 435-44.
2. Zhang, D et al. Proceedings of the National Academy of Sciences of the United States of America vol. 98,10 (2001): 5746-51.
3. Trapani, J A et al. The Journal of biological chemistry vol. 271,8 (1996): 4127-33.