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Recombinant Human DR4 protein (rFc Tag) (HPLC verified)

种属

Human

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg4600

Print datasheet

Synonyms

APO2, CD261, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TNFRSF10A

验证数据展示

  • Purity of Recombinant Human DR4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human DR4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human DR4 was greater than 90% as determined by SEC-HPLC.

    The purity of Human DR4 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human DR4 protein Ala109-Asn239 (Accession# O00220) with a rabbit IgG Fc tag at the C-terminus.
基因ID 8797
蛋白编号 O00220
预测分子量 40.6 kDa
SDS-PAGE 40-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

DR4, also known as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), is a member of the TNF superfamily. TRAIL activates apoptosis through the death receptors DR4 (also known as TRAILR1 and TNFRSF10A) and DR5 (also known as TRAILR2, KILLER and TNFRSF10B). DR4 and DR5 are single-pass type I membrane proteins that contain intracellular death domains (DD) and upon activation mediate apoptotic signals. Binding of TRAIL to DR4 or DR5 results in receptor oligomerization and recruitment of FAS-associated protein with death domain (FADD) and caspase 8 to form a functional death-inducing signalling complex (DISC). Upon DISC formation, caspase 8 is cleaved and activated, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. DR4 or DR5 promotes the activation of NF-kappa-B and play an important role in inflammation.

参考文献:

1. Ozawa F. et al. (2001). Cancer Lett. 163(1):71-81.
2. Johnstone RW. et al. (2008). Nat Rev Cancer. 8(10):782-798.
3. Chaudhary PM. et al. (1997). Immunity. 7(6):821-830.
4. Tang W. et al. (2009). Cell Res. 19(6):758-767.