Recombinant Human Component C9 protein (His Tag)
种属
Human
纯度
>80 %, SDS-PAGE
标签
His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >80 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Component C9 protein Gln22-Lys559 (Accession# P02748) with a His tag at the C-terminus. |
| 基因ID | 735 |
| 蛋白编号 | P02748 |
| 预测分子量 | 62.1 kDa |
| SDS-PAGE | 65-75 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Component C9 is the final pivotal protein in the membrane attack complex (MAC) of the complement system, an essential part of innate immunity. It is a pore-forming molecule that, upon sequential binding to the C5b-8 complex embedded in a pathogen's cell membrane, undergoes a profound conformational change. Multiple C9 monomers polymerize to form a cylindrical pore, which fully penetrates the lipid bilayer. This creates a functional channel that allows the uncontrolled flux of ions and water, leading to osmotic lysis and the direct destruction of targeted cells, such as bacteria. Thus, C9 acts as the critical executioner molecule in the complement's cytolytic pathway, providing a direct mechanism for eliminating microbial threats.
参考文献:
1. Fu, Xiaoyan et al. Scientific reports vol. 6 (2016): 30239. 2. Chang, C P et al.The Journal of biological chemistry vol. 269,42 (1994): 26424-30.
