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Recombinant Human CD55 protein (rFc Tag)(HPLC verified)

种属

Human

纯度

>90 %, SDS-PAGE
>90%, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg4246

Print datasheet

Synonyms

Complement decay-accelerating factor, CR, CROM, DAF, TC

验证数据展示

  • Purity of Recombinant Human CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CD55 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CD55 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90%, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human CD55 protein Asp35-Ser353 (Accession# P08174) with a rabbit IgG Fc tag at the C-terminus.
基因ID 1604
蛋白编号 P08174
预测分子量 61.2 kDa
SDS-PAGE 65-80 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD55, also known as decay-accelerating factor (DAF), is a membrane lipid microstructural domain-associated GPI-anchored protein with a molecular weight ranging from 50 to 100 kDa depending on the cell type. CD55 protects cells from complement-mediated attack by accelerating the decay of C3 and C5, and its absence has been linked to a number of pathologies resulting from over-activation of the complement system. CD55 can signal within cells to promote malignant transformation, cell survival and inhibit apoptosis. CD55 can signal intracellularly to promote malignant transformation, cancer progression, cell survival, angiogenesis, and inhibition of apoptosis. CD55 is enriched in cancer stem cell (CSC) ecosystems in a variety of tumors including breast, ovarian, and cervical cancers and can be induced by chemotherapy and hypoxic environments. (PMID: 36216147)

参考文献:

1. Bharti R,et al. Cancer Lett. 2022;551:215935.                                                         
2. González-Del-Barrio L, et al. FASEB J. 2023;37(11):e23256.                                  
3. Kurolap A, et al. Hum Genet. 2023;142(5):683-690.