Speedy™ Human CD48 One-Step ELISA Kit
Sensitivity
8.9 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : SE50104
Synonyms
BLAST, BLAST1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 molecule
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD48 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 8.9 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 962 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 90%-112% |
| Cell culture supernatant | 90% | 86%-95% |
| Cell lysate | 90% | 83%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,305.3 | 29.6 | 2.3 |
| 2 | 8 | 326.0 | 10.7 | 3.3 |
| 3 | 8 | 166.6 | 2.4 | 1.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,298.3 | 39.4 | 3.0 |
| 2 | 16 | 324.8 | 12.0 | 3.7 |
| 3 | 16 | 163.0 | 4.7 | 2.9 |
Background Information
CD48, also known as BLAST-1 or BCM-1, is a member of the signaling lymphocyte activation molecule (SLAM) family and is involved in immune cell adhesion and activation. It is a glycosylphosphatidylinositol (GPI) -anchored cell surface glycoprotein that interacts with various cell surface receptors, including CD244 (2B4) and CD2. CD48 plays a crucial role in regulating immune responses by mediating cell-cell interactions and signaling pathways that influence T cell activation, NK cell function, and the formation of immune synapses. Soluble CD48 (sCD48) is detected in human serum and plasma. sCD48 may serve as a potential therapeutic agent for inflammatory diseases by modulating immune cell activation and cytokine signaling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BLAST, BLAST1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 molecule |
