Human CD39/ENTPD1 ELISA Kit
Sensitivity
1.1 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00603 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD39/ENTPD1 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD39/ENTPD1. The assay recognizes human CD39/ENTPD1. An antibody specific for human CD39/ENTPD1 has been pre-coated onto the microwells. The human CD39/ENTPD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD39/ENTPD1 is added to detect the captured human CD39/ENTPD1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD39/ENTPD1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 953 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 81%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 625.1 | 14.9 | 2.4 |
| 2 | 8 | 154.1 | 2.7 | 1.7 |
| 3 | 8 | 74.8 | 2.4 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 603.4 | 25.6 | 4.2 |
| 2 | 16 | 156.6 | 3.7 | 2.4 |
| 3 | 16 | 76.6 | 3.5 | 4.6 |
Background Information
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1), also known as CD39 or NTPDase 1, is a cell surface enzyme that plays a crucial role in regulating extracellular nucleotide levels and immune responses. ENTPD1 is highly expressed on regulatory T cells (Tregs), where it contributes to their suppressive functions by depleting extracellular ATP and generating adenosine. It is a noncovalent tetrameric protein, and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers. Moreover, ENTPD1 is involved in the regulation of dendritic cell function and T cell activation. It modulates the local concentration of nucleotides and nucleosides, which in turn affects immune cell signaling and activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39 |