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Recombinant Human BAFF/TNFSF13B protein (hFc Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 0.6-2.4 ng/mL

Cat no : Eg0155

Print datasheet

Synonyms

TNFSF13B, B cell activating factor, B lymphocyte stimulator, BAFF, B-cell-activating factor

验证数据展示

  • Purity of Recombinant Human BAFF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human BAFF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.

    Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.
来源 HEK293-derived Human BAFF protein Ala134-Leu285 (Accession# Q9Y275-1) with a human IgG1 Fc tag at the N-terminus.
基因ID 10673
蛋白编号 Q9Y275-1
预测分子量 43 kDa
SDS-PAGE 45-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

TNFSF13B, also known as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. This cytokine is expressed in B lineage cells, and acts as a potent B cell activator. The BAFF system promotes B cell survival and differentiation and plays a prominent role in the pathogenesis of autoimmune diseases. It has been shown that BAFF inhibitors are in clinical trials for systemic lupus erythematosus with significant efficacy.

参考文献:

1. Cristian R Smulski et al (2018). Front Immunol. Oct 8;9:2285.
2. Edina Schweighoffer et al (2021).  Curr Opin Immunol. Aug;71:124-131.
3. Anne Davidson. (2010). Curr Opin Immunol. Dec;22(6):732-9.
4. Shingo Nakayamada et al (2016).Inflamm Regen. Jul 21;36:6.