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Recombinant Human BAFF/TNFSF13B protein (hFc Tag)

ED50

0.6-2.4 ng/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

10673

Accession

Q9Y275-1

Cat No : Eg0155

Print datasheet

Synonyms

BAFF, BLYS, CD257, DTL, TALL1, TALL-1, THANK, TNFSF13B, TNFSF20, ZTNF4

验证数据展示

  • Purity of Recombinant Human BAFF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human BAFF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.

    Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human BCMA (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human BAFF (hFc tag) with a linear range of 0.6-2.4 ng/mL.
Source HEK293-derived Human BAFF protein Ala134-Leu285 (Accession# Q9Y275-1) with a human IgG1 Fc tag at the N-terminus.
Predicted Molecular Mass 43 kDa
SDS-PAGE 45-50 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFSF13B, also known as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. This cytokine is expressed in B lineage cells, and acts as a potent B cell activator. The BAFF system promotes B cell survival and differentiation and plays a prominent role in the pathogenesis of autoimmune diseases. It has been shown that BAFF inhibitors are in clinical trials for systemic lupus erythematosus with significant efficacy.

References:

1. Cristian R Smulski et al (2018). Front Immunol. Oct 8;9:2285.
2. Edina Schweighoffer et al (2021).  Curr Opin Immunol. Aug;71:124-131.
3. Anne Davidson. (2010). Curr Opin Immunol. Dec;22(6):732-9.
4. Shingo Nakayamada et al (2016).Inflamm Regen. Jul 21;36:6.