Speedy™ Human ADAMTS13 One-Step ELISA Kit
Sensitivity
27.8 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human ADAMTS13 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 27.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11093 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 109% | 89%-127% |
| Cell culture supernatant | 98% | 93%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,476.5 | 60.1 | 2.4 |
| 2 | 8 | 631.0 | 14.0 | 2.2 |
| 3 | 8 | 315.4 | 5.3 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,403.2 | 100.1 | 4.2 |
| 2 | 16 | 608.2 | 25.7 | 4.2 |
| 3 | 16 | 309.0 | 14.9 | 4.8 |
Background Information
A disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) is a multidomain metalloprotease for which until now only a single substrate has been identified. ADAMTS13 cleaves the polymeric force-sensor von Willebrand factor (VWF) that unfolds under shear stress and recruits platelets to sites of vascular injury. In patients with immune-mediated thrombotic thrombocytopenic purpura (iTTP), a rare life threatening disease, ADAMTS13 is targeted by autoantibodies that inhibit its activity or promote its clearance. In the absence of ADAMTS13, VWF polymers are not adequately processed, resulting in spontaneous adhesion of blood platelets, which presents as severe, life-threatening microvascular thrombosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADAM TS 13, ADAM TS13, ADAMTS 13, C9orf8, TTP |
